Bee-derived antibacterial peptide, defensin-1, promotes wound re-epithelialisation in vitro and in vivo

Bee-derived antibacterial peptide, defensin-1, promotes wound re-epithelialisation in vitro and in vivo

Blog Article

Abstract Royal jelly (RJ) has successfully been used as a remedy in wound healing.RJ has multiple effects, including antibacterial, anti-inflammatory and immunomodulatory activities, in various cell types.However, no component(s) (other than antibacterial) have been identified in RJ-accelerated wound healing.

In this study, we demonstrate that keratinocytes are responsible for the elevated production of matrix metalloproteinase-9 (MMP-9) after incubation with a water extract of RJ.Furthermore, the keratinocyte migration and wound closure rates were significantly increased in the presence of RJ extract.MMP-9 production was reduced significantly following proteinase K treatment but remained stable after Kratom Gummies heat treatment, indicating that active component(s) have a proteinous character.

To identify the component responsible for inducing MMP-9 production, RJ extract was fractionated using C18 RP-HPLC.In fractions exhibiting stimulatory activity, we immunochemically detected the bee-derived antibacterial peptide, defensin-1.Defensin-1 was cloned, Hemp Wicks and recombinant peptide was produced in a baculoviral expression system.

Defensin-1 stimulated MMP-9 secretion from keratinocytes and increased keratinocyte migration and wound closure in vitro.In addition, defensin-1 promoted re-epithelisation and wound closure in uninfected excision wounds.These data indisputably demonstrate that defensin-1, a regular but concentration variable factor found in honey and RJ, contributes to cutaneous wound closure by enhancing keratinocyte migration and MMP-9 secretion.